Journal: Journal of Translational Medicine
Article Title: TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury
doi: 10.1186/s12967-023-04027-4
Figure Lengend Snippet: TREM-1 activation promotes mitochondrial fragmentation and mitophagy of macrophages. A Mitochondrial morphology stained with TOM20. B – C The morphological skeleton and quantification of mitochondrial morphology. D Dnm1 , Mff1 , Mtfp1 , Mfn1 , Mfn2, and Opa1 mRNA. E – F Protein levels of TOM20, DRP1, MFN2 and OPA1. G – I PGAM5, and MTFP1 protein. J-K Pink1, Beclin1, and LC3 protein
Article Snippet: Rabbit-anti-MTFP1 antibody , Proteintech , 14257-1-AP , 1:1000.
Techniques: Activation Assay, Staining